Evaluation of Anti Microbial Activity and Synthesis of Silver Nanoparticles from Hibiscus Cannabinus and Punica Granatum

S Karthik, A.I Thirupathamma, A Nagendhiran

Abstract


Now-a-days the use of herbs is gaining importance and the medicinal plants can be exploited in different fields. The present study has attempted on the phytochemicals and antibacterial property of two plants Hibiscus cannabinus and Punica granatum. Hibiscus cannabinus was found to be positive results for flavonoids, saponins, tannins and terpenoids. Punica granatum was found to be positive results for sterols and terpenoids. The antimicrobial properties of those extracts were investigated using disc diffusion method. The organic solvents such as acetone, chloroform and ethanol were employed to extract the antimicrobial components. The antimicrobial activity was tested against four pathogenic microbial strains such as E. coli, Klebsiella Sp., Pseudomonas Sp. and Staphylococcus Sp. which included both gram negative and gram positive organisms. All extract of the plants apparently showed considerable inhibitory action against all the tested microorganisms. Today eco-friendly method for synthesis of nanoparticles gaining significant importance. In this context, the role of plants in the reduction to silver nanoparticles has been analysed using the plants under study. The plant Punica granatum found to have reducing ability and had synthesized Silver nanoparticles (AgNP’s) but the time taken was longer. The silver nanoparticles thus produced showed characteristic peak in UV-Visible spectra and also possessed good antimicrobial property which can be further exploited in different fields prone to bacterial infections.


Keywords


Phytochemicals, Hibiscus cannabinus, Punica granatum, Antimicrobial activity, Silver nanoparticles.

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